一、基本信息
卢争辉,博士,副教授,硕士研究生导师
电子邮件:zhenghuilu(at)hubu.edu.cn
研究领域:合成生物学、生物传感、分子酶学
二、教育背景
2015.09–2018.06 九游会老哥俱乐部,微生物学,博士
2012.09–2015.06 九游会老哥俱乐部,微生物学,硕士
2008.09–2012.06 九游会老哥俱乐部,生物工程,学士
三、工作简历
2021.10至今 九游会老哥俱乐部,副教授
2020.07–2021.09 九游会老哥俱乐部,讲师
2018.07–2020.06 九游会老哥俱乐部,微生物学,博士后
四、科研项目
1. 国家自然科学基金青年项目(31901031):感受态形成促进串联基因复制的分子机理,2020–2022年,25万
2. 中国博士后科学基金面上项目(2019M662571):转录因子ComK调控枯草杆菌胁迫压力应答的分子机理研究,2019-2020年,8万
3. 湖北省自然科学基金面上项目(青年)(2019CFB176):H100Q 引起碱性木聚糖酶 Xyn10A 碱耐受性改变的机制研究,2019–2020年,5万
4. 国家自然科学基金委员会与英国皇家学会合作交流项目(IEC\NSFC\181305):通过小角X射线散射和中子散射(SAXS-SANS)研究N-端结构域影响淀粉酶Amy703钙离子依赖性的机制,2020.01–2022.12,9.9万(排序第三)
五、代表著作(#共同第一作者,*通讯作者)
1. Lu Z, Yang S, Yuan X, Shi Y, Ouyang L, Jiang S, Yi L, Zhang G*. (2019) CRISPR-assisted multi-dimensional regulation for fine-tuning gene expression in Bacillus subtilis. Nucleic acids research 47, e40–e40.
2. Lu Z, Li X, Zhang R, Yi L, Ma Y, Zhang G*. (2019) Tunnel engineering to accelerate product release for better biomass-degrading abilities in lignocellulolytic enzymes. Biotechnology for Biofuels 12, 1–9.
3. Lu Z, Hu X, Shen P, Wang Q, Zhou Y, Zhang G*, Ma Y. (2018) A pH-stable, detergent and chelator resistant type I pullulanase from Bacillus pseudofirmus 703 with high catalytic efficiency. International Journal of Biological Macromolecules 109, 1302–1310.
4. Wang X#, Lu Z#, Xu T, Selvaraj JN, Yi L, Zhang G*. (2018) Improving the specific activity and thermo-stability of alkaline pectatelyase from Bacillus subtilis 168 for bioscouring. Biochemical Engineering Journal 129, 74–83.
5. Lu Z, Wang Q, Jiang S, Zhang G*, Ma Y. (2016) Truncation of the unique N-terminal domain improved the thermos-stability and specific activity of alkaline α-amylase Amy703. Scientific Reports 6, 22465.
6. Lu Z, Tian C, Li A, Zhang G*, Ma Y. (2014) Identification and characterization of a novel alkaline α-amylase Amy703 belonging to a new clade from Bacillus pseudofirmus. Journal of Industrial Microbiology & Biotechnology 41, 783–793.
7. Li C, Jiang S, Chao Du, Lu Z, Nisha He, Zhou Y, ...Jiang S*, Zhang G*. (2021) High-level extracellular expression of a new β-N-acetylglucosaminidase in Escherichia coli for producing GlcNAc. Frontiers in Microbiology 12, 478.
8. Wu P, Luo F, Lu Z, Zhan Z, Zhang G*. (2020) Improving the catalytic performance of pectate lyase through pectate lyase/Cu3(PO4)2 hybrid nanoflowers as an immobilized enzyme. Frontiers in Bioengineering and Biotechnology 8, 280.
9. Zhou Y, Lu Z, Wang X, Selvaraj JN, Zhang G*. (2018) Genetic engineering modification and fermentation optimization for extracellular production of recombinant proteins using Escherichia coli. Applied Microbiology and Biotechnology 102, 1545–1556.
10. Wang S, Wang Q, Zhou Y, Lu Z, Zhang G*, Ma Y. (2017) A new GH13 α-glucosidase from alkaliphilic Bacillus pseudofirmus 703 with both exo-α-l,4-glucosidase and oligo-l,6-glucosidase activities towards amylopectin. International Journal of Biological Macromolecules 101, 973–982.
11. 卢争辉,周玉玲,张晓舟,张桂敏*。感受态还是芽胞?细胞命运决定的遗传调控网络。生物工程学报,2015(11): 1543-1552.
12. 李信志,卢争辉,周玉玲,李世宇,张桂敏*。枯草芽孢杆菌SCK6超级感受态的制备和转化条件优化。生物工程学报, 201733(4): 692-698.
13. 望潇文,向腊,徐婷,卢争辉,张桂敏*。碱性果胶酶高产菌株的构建和高密度发酵,生物工程学报,2017, 33(12): 2017-2027.
六、授权专利
1. 张桂敏,卢争辉,沈盼盼,周玉玲,马延和。针对淀粉酶BLA的启动子文库及强启动子。专利授权号:ZL201710956947.3
2. 张桂敏,卢争辉,袁芯,彭文舫,石云云,蒋思婧,马延和。一种特异性调节枯草芽孢杆菌外源基因表达的dcas9-ω融合蛋白及其应用。专利授权号:201710965231.X
3. 张桂敏,卢争辉,周玉玲,马延和。一种启动子文库构建方法和强启动子。专利授权号:ZL2015107992498
4. 张桂敏,卢争辉,马延和。一种热稳定性及比酶活提高的碱性淀粉酶突变体。专利授权号:ZL201410852880.5
七、获奖
1. “废弃物发酵制备饲用微生态制剂关键技术及产业化”获2018年湖北省科技进步三等奖(张桂敏,谌颉,易犁,凌华云,周玉玲,邱权,卢争辉,张东晓,戴小方)
2. 第十一届中国酶工程学术研讨会“青年优秀报告奖”