一、基本情况
印文，博士，副教授，硕士生导师
电子邮件：yinwen@hubu.edu.cn
研究领域：生物传感器开发、细菌第二信使分子调控、肿瘤细胞的精准靶向及治疗等方面的研究
社会兼职：中国微生物学会会员、湖北省合成生物学学会会员
二、教育背景
2012.09-2017.12 中国科学院大学，微生物学，理学博士
2008.09-2011.06 华中农业大学，微生物学，理学硕士
2004.09-2008.06 湖北文理学院，生物科学，理学学士
三、工作经历
2022.11至今 湖北大学，九游会老哥俱乐部，讲师、副教授
2018.02-2022.11 华中农业大学，生命科学技术学院，博士后
2011.08-2012.08 武汉友芝友生物制药有限公司，高级研究助理
四、科研项目
1. 国家自然科学基金青年项目，32000055，氮代谢调控因子GlnR通过cda操纵子调控细胞渗透平衡的新机制，2021.01-2023.12，24万元，主持
2. 中国博士后科学基金面上项目一等资助，2018M630872，c-di-AMP通过核糖开关调控Kdp钾离子转运系统的机制，2018.06-2020.06，8万元，主持
3. 中国科学院高致病性病原生物学与生物安全重点实验室开放研究基金，2019SPCAS001，用于肺炎链球菌感染控制的嵌合裂解酶ClyJ的功能研究及改造，2019.01-2020.12，6万元，主持
4. 国家自然科学基金面上项目，32171424，基于第二信使c-di-AMP的近红外光控基因表达调控系统的构建与应用，2022.01-2025.12，59万元，参与
5. 国家自然科学基金面上项目，31971339，用于双重转录调控的茶碱响应型生物元件的挖掘与改造，2020.01-2023.12，56万元，参与
6. 国家自然科学基金面上项目，31970074，苏云金芽胞杆菌中Hfq同时调节c-di-GMP与c-di-AMP信号系统的分子机制，2020.01-2023.12，58万元，参与
7. 国家自然科学基金重大研究计划，91743108，大气细颗粒物与流感病毒相互作用及致毒效应研究，2018.01-2020.12，80万元，参与
五、代表性论文（#并列第一作者，*通讯作者）
1. Wen Yin#, Leyao Li#, Yang Yang#, Yuxin Yang, Ruijing Liang, Lixin Ma, Junbiao Dai, Guobin Mao*, Yingxin Ma*. Ultra-sensitive detection of the SARS-CoV‑2 nucleocapsid protein via a clustered regularly interspaced short palindromic repeat Cas12a-mediated immunoassay. ACS Sensors, 2024, 9(6): 3150-3157. (Cover Story)
2. Guobin Mao*, Xing Luo, Silu Ye, Xun Wang, Jin He, Jilie Kong, Junbiao Dai, Wen Yin*, Yingxin Ma*. Fluorescence and Colorimetric Analysis of African Swine Fever Virus Based on the RPA-Assisted CRISPR/Cas12a Strategy. Analytical Chemistry, 2023, 95: 8063-8069. (Cover Story)
3. Wen Yin, Ji Hu, Fang Chen, Li Zhu, Yingxin Ma, Nuo Wang, Hongping Wei, Hang Yang, Shan-Ho Chou, Jin He*. Combining hybrid nanoflowers with hybridization chain reaction for portable and sensitive detection of SARS-CoV-2. Analytica Chimica Acta, 2023, 1279: 341838.
4. Wen Yin, Li Zhu, Hui Xu, Qing Tang, Yingxin Ma, Shan-Ho Chou, Jin He*. Bio-hybrid nanoarchitectonics of nanoflower-based ELISA method for the detection of Staphylococcus aureus. Sensors and Actuators: B. Chemical, 2022, 366: 132005.
5. Wen Yin, Xia Cai, Hongdan Ma, Li Zhu, Yuling Zhang, Shan-Ho Chou, Michael Y. Galperin, Jin He*. A decade of research on the second messenger c-di-AMP. FEMS Microbiology Reviews, 2020, 44(6): 701-724.
6. Jin He#,*, Wen Yin#, Michael Y. Galperin*, Shan-Ho Chou*. Cyclic di-AMP, a second messenger of primary importance: tertiary structures and binding mechanisms. Nucleic Acids Research, 2020, 48(6): 2807-2829.
7. Wen Yin#, Wei Li#, Qin Li, Yuanyuan Liu, Ji Liu, Min Ren, Yingxin Ma, Zhiping Zhang, Xiaowei Zhang, Yuntao Wu, Shibo Jiang, Xian-En Zhang, Zongqiang Cui*. Real-time imaging of individual virion-triggered cortical actin dynamics for human immunodeficiency virus entry into resting CD4 T cells. Nanoscale, 2020, 12(1): 115-129. (Cover Story)
8. Wen Yin#, Siyang Xu#, Yiting Wang, Yuling Zhang, Shan-Ho Chou, Michael Y. Galperin, Jin He*. Ways to control harmful biofilms: Prevention, inhibition, and eradication. Critical Reviews in Microbiology, 2021, 47(1): 57-78.
9. Siyang Xu#, Wen Yin#, Yuling Zhang, Qimei Lv, Yijun Yang, Jin He*. Foes or friends? Bacteria enriched in the tumor microenvironment of colorectal cancer. Cancers, 2020, 12(2): 372.
10. Wen Yin, Yiting Wang, Lu Liu, Jin He*. Biofilms: the microbial “protective clothing” in extreme environments. International Journal of Molecular Sciences, 2019, 20(14): 3423. (Highly Cited Paper)
11. Wen Yin, Lu Liu, Siyang Xu, Jin He*. (2020) Cyclic di-GMP signaling systems in the Gram-positive Bacillus cereus group. In: Shan-Ho Chou, Nicolas Guiliani, Vincent T. Lee, Ute Römling (eds) Microbial cyclic di-nucleotide signaling. Chapter 16. Springer, Cham, pp 261-275.
12. Wen Yin, Wei Li, Qin Li, Chong Qin, Zhiping Zhang, Xian-En Zhang*, Zongqiang Cui*. Structural characterization and analysis of human immunodeficiency virus encapsulating quantum dots. Journal of Nanoscience and Nanotechnology, 2018, 18(7): 4487-4494.
13. Qin Li, Wen Yin, Wei Li, Zhiping Zhang, Xiaowei Zhang, Xian-En Zhang, Zongqiang Cui*. Encapsulating quantum dots within HIV-1 virions through site-specific decoration of the matrix protein enables single virus tracking in live primary macrophages. Nano Letters, 2018, 18(12): 7457-7468.
14. Qin Li#, Wei Li#, Wen Yin, Jia Guo, Zhi-Ping Zhang, Dejun Zeng, Xiaowei Zhang, Yuntao Wu*, Xian-En Zhang*, Zongqiang Cui*. Single-particle tracking of human immunodeficiency virus type 1 productive entry into human primary macrophages. ACS Nano, 2017, 11(4): 3890-3903.
15. Guobin Mao#, Silu Ye#, Wen Yin, Yang Yang, Xinghu Ji, Jin He, Yingxia Liu, Junbiao Dai, Zhike He*, Yingxin Ma*. Ratiometric fluorescent Si-FITC nanoprobe for immunoassay of SARS-CoV-2 nucleocapsid protein. Nano Research, 2023, 16, 2859-2865. (Cover Story)
16. Chong Qin, Wei Li, Qin Li, Wen Yin, Xiaowei Zhang, Zhi-Ping Zhang, Xian-En Zhang, Zongqiang Cui*. Real-time dissection of dynamic uncoating of individual influenza viruses. Proceedings of the National Academy of Sciences of the United States of America, 2019, 116(7): 2577-2582.
17. Xun Wang, Xia Cai, Hongdan Ma, Wen Yin, Li Zhu, Xinfeng Li, Heon M. Lim, Shan-Ho Chou, Jin He*. A c-di-AMP riboswitch controlling kdpFABC operon transcription regulates the potassium transporter system in Bacillus thuringiensis. Communications Biology, 2019, 2: 151.
18. Yingxin Ma#, Guobin Mao#, Weiren Huang#, Guoqiang Wu, Wen Yin, Xinghu Ji, Zishi Deng, Zhiming Cai, Xian-En Zhang, Zhike He*, Zongqiang Cui*. Quantum dot nanobeacons for single RNA labeling and imaging. Journal of the American Chemical Society, 2019, 141(34): 13454-13458.
19. Zhaoqing Yu#, Yang Fu#, Wei Zhang, Li Zhu, Wen Yin, Shan-Ho Chou, Jin He*. The RNA chaperone protein Hfq regulates the characteristic sporulation and insecticidal activity of Bacillus thuringiensis. Frontiers in Microbiology, 2022, 13: 884528.
20. Wei Li#, Ji Liu#, Yuanyuan Liu, Qin Li, Wen Yin, Kevin K Wanderi, Xiaowei Zhang, Zhiping Zhang, Xian-En Zhang, Zongqiang Cui*. HIV-1 uses dynamic podosomes for entry into macrophages. Journal of Virology, 2021, 95(10): e02480-20.
21. Yang Fu#, Zhaoqing Yu#, Li Zhu, Zhou Li, Wen Yin, Xiaodong Shang, Shan-Ho Chou, Qi Tan*, Jin He*. The multiple regulatory relationship between RNA-chaperone Hfq and the second messenger c-di-GMP. Frontiers in Microbiology, 2021, 12, 689619.
22. Cao Zheng, Zhaoqing Yu, Cuiying Du, Yujing Gong, Wen Yin, Xinfeng Li, Zhou Li, Ute Römling, Shan-Ho Chou, Jin He*. 2-Methylcitrate cycle: a well-regulated controller of Bacillus sporulation. Environmental Microbiology, 2020, 22(3): 1125-1140.
23. Hang Yang, Dehua Luo, Irina Etobayeva, Xiaohong Li, Yujing Gong, Shujuan Wang, Qiong Li, Poshi Xu, Wen Yin, Jin He, Daniel Nelson, and Hongping Wei*. Linker editing of pneumococcal lysin ClyJ conveys improved bactericidal activity. Antimicrobial Agents and Chemotherapy, 2020, 64(2): e01610-19.
六、专利
1. 崔宗强，印文，李炜，张先恩，李芹，刘霁。一种抑制肌动蛋白重组的多肽及应用。专利号：ZL2018101598383
七、荣誉与获奖
1. 第九届全国大学生生命科学竞赛（科学探究类）指导教师二等奖
2. 九游会老哥俱乐部第五届青年教师教学竞赛一等奖
