一、基本信息
陈纯琪，博士，教授，博士生导师
电子邮件：ccckate0722(AT)hubu.edu.cn
研究方向：免疫学、微生物学、结构生物学与酶学
研究领域：结构生物学、酶应用与改造、酶的机理研究。酶是所有生命活动的发动机，酶的结构与催化机理研究对于了解生物基本运作、疾病发生原因、药物开发、以及医药产业与绿色生物工艺发展具有重大意义。陈纯琪教授通过结构生物学方法(X光晶体学与冷冻电镜)解析酶的三维结构与底物结合的复合体结构，阐释催化机理，并开展基于结构的理性设计，改造酶的特征与活性。工作内容主要涵盖三个体系：(1) 新型萜类合成酶；(2) 天然与人造合成塑料降解酶；(3) 霉菌毒素分解酶。目前已在Nature, Nature Reviews Chemistry, Nature Catalysis, Immunity, Nature Communications, Angew. Chem. Intl. Ed., JACS, ACS Catalysis等SCI期刊发表论文近100篇，共21篇获选为封面文章，申请国内外专利26个，已授权16个。2020年获“湖北省楚天学者特聘教授”、“湖北省百人计划特聘专家”、“湖北省创新群体”、“天津市自然科学二等奖”(第二完成人)等。
二、教育背景
2018.06至今 九游会老哥俱乐部，教授
2014.01–2018.5 中国科学院天津工业生物技术研究所，访问学者，高级台湾青年访问学者
三、工作经历
2012.01–2013.12 中国科学院微生物研究所，副研究员，高级台湾青年访问学者
2008.02–2011.12 台湾中研院特聘博士后
2003.09–2008.01 台湾大学，微生物学研究所，博士
1999.09–2001.07 台湾大学，医事技术学研究所，硕士
1995.09–1999.07 台湾大学，医事技术学系，学士
四、承担科研项目情况
1. 国家自然科学基金，面上项目，32371307，新型倍半萜环化酶家族结构与机理研究，2024-01至2027-12，50万，在研，主持；
2. 国家重点研发计划，绿色生物制造专项，2021YFC2100302，医药与食品工业酶创制与催化，2021-07至2024-06，68.7万，在研，子课题负责人；
3. 国家自然科学基金，面上项目，31971205，新型倍半萜合成酶晶体结构与催化机理研究，2020-01至2023-12，58万，在研，主持；
4. 国家自然科学基金，青年基金，31200677，免疫球蛋白样转物3受体鉴定：功能性以及结构性研究，2013-01至2015-12，25万，已结题，主持。
五、代表性论文
1. Yang, Y.,# Cheng, S.,# Zheng, Y.,# Xue, T., Huang, J.-W., Zhang, L., Yang, Y., Guo, R.-T.,* Chen, C.-C.* Remodeling the polymer-binding cavity to improve the efficacy of PBAT-degrading enzyme. Journal of Hazardous Materials, 2023, Accepted. https://doi.org/10.1016/j.jhazmat.2023.132965
2. Li, H.,# Huang, J.-W.,# Dai, L.,# Zheng, H., Dai, S., Zhang, Q., Yao, L., Yang, Y., Yang, Y., Min, J., Guo, R.-T.,* Chen, C.-C.* The structural and functional investigation into an unusual nitrile synthase. Nature Communications, 2023, Accepted.
3. Yuan,L.,# Ma, X.,# Yang, Y.,# Qu, Y., Li, X., Zhu, X., Ma, W., Duan, J., Xue, J., Yang, H., Huang, J.-W., Yi, S., Zhang, M., Cai, N., Zhang, L., Ding, Q., Lai, K., Liu, C., Zhang, L., Liu, X., Yao, Y., Zhou, S., Li, X., Shen, P., Chang, Q., Malwal, S. R., He, Y., Li, W., Chen, C., Chen, C.-C., Oldfield, E., Guo, R.-T.,* Zhang, Y.* Phosphoantigens glue butyrophilin 3A1 and 2A1 to activate Vγ9Vδ2 T cells. Nature, 2023, 621(7980): 840-848.
4. Dai, L.,# Niu, D.,# Huang, J.-W.,# Li, X., Shen, P., Li, H., Xie, Z., Min, J., Hu, Y., Yang, Y., Guo, R.-T.,* Chen, C.-C.,* Cryo-EM structure and rational engineering of a superefficient ochratoxin A-detoxifying amidohydrolase. Journal of Hazardous Materials, 2023, 458: 131836.
5. Li, X.,# Shi, B.,# Huang, J.-W., Zeng, Z., Yang, Y., Zhang, L., Min, J., Chen, C.-C.,* Guo, R.-T.* Functional tailoring of a PET hydrolytic enzyme expressed in Pichia pastoris. Bioresources and Bioprocessing, 2023, 10(1): 26.
6. Xin, L.,# Min, J.,# Hu, H.,# Li, Y.,# Du, C., Xie, B., Cheng, Y., Deng, X., Deng, X., Shen, K., Huang, J., Chen, C.-C., Guo, R.-T.*, Dong, C.,*, Zhou, H.-B.* Structure-guided identification of novel dual-targeting estrogen receptor α degraders with aromatase inhibitory activity for the treatment of endocrine-resistant breast cancer. European Journal of Medicinal Chemistry, 2023, 253: 115328.
7. Yang, Y.,# Min, J.,# Xue, T.,# Jiang, P., Liu, X., Peng, R., Huang, J.-W., Qu, Y., Li, Xian., Ma, Ning., Tsai, F.-C., Dai, L., Zhang, Q., Liu, Y.,* Chen, C.-C.,* Guo, R.-T.* Complete bio-degradation of poly(butylene adipate-co-terephthalate) via engineered cutinases. Nature Communications, 2023, 14(1): 1645.
8. Li, A.,# Wang, Q.,# Song, X.,# Zhang, X., Huang, J.-W., Chen, C.-C., Guo, R.-T., * Wang, B.,* Reetz, M.T.* Engineering of a P450-based Kemp eliminase with a new mechanism. Chinese Journal of Catalysis, 2023, 47: 191-199.
9. Zhang, X.,# Shen, P.,# Zhao, J.,# Chen, Y., Li, X., Huang, J.-W., Zhang, L., Li, Q., Gao, C., Xing, Q., Chen, C.-C., Guo, R.-T.,* Li, A.* Rationally controlling selective steroid hydroxylation via scaffold sampling of a P450 Family. ACS Catalysis, 2023, 13(2): 1280-1289.
10. Chen, Q.,# Li, B.,# Zhang, L.,# Chen, X., Zhu, X., Chen, F., Shi, M., Chen, C.-C., Yang, Y., Guo, R.-T.,* Liu, W., Xu, J., Zheng, G.* Engineered imine reductase for larotrectinib intermediate manufacture. ACS Catalysis, 2022, 12 (23): 14795-14803.
11. Dai, L.,# Li, H.,# Huang, J.-W.,# Hu, Y.,# He, M., Yang, Y., Min, J., Guo, R.-T.,* Chen, C.-C.* Structure-based rational design of a short-chain dehydrogenase/reductase for improving activity toward mycotoxin patulin. International Journal of Biological Macromolecules, 2022, 222: 421-428.
12. Wu, L.,# An, J.,# Jing, X.,# Chen, C.-C., Dai, L., Liu, W.,* Guo, R.-T.,* Nie, Y.* Molecular insights into the regioselectivity of the Fe(II)/2-ketoglutarate-dependent dioxygenase-catalyzed C-H hydroxylation of amino acids. ACS Catalysis, 2022, 12(19): 11586-11596.
13. Zhang, L.,# Zhang, X.,# Min, J.,# Liu, B., Huang, J.-W., Yang, Y., Liu, W., Dai, L., Yang, Y., Chen, C.-C.,* Guo, R.-T.* Structural insights to a bi-functional isoprenyl dihposphate synthase that can catalyze head-to-tail and head-to-middle condensation. International Journal of Biological Macromolecules, 2022, 214: 492-499.
14. Li, H.,# Yang, Y.,# Hu, Y.,# Chen, C.-C., Huang, J.-W., Min, J., Dai, L.,* Guo, R.-T.* Structural analysis and engineering of aldo-keto reductase from glyphosate-resistant Echinochloa colona. Journal of Hazardous Materials, 2022, 436: 129191.
15. Malwal, S. R.,# Shang, N.,# Liu, W., Li, X., Zhang, L., Chen, C.-C., Guo, R.-T.,* Oldfield, E.* A structural and bioinformatics investigation of a fungal squanlene synthase and comparisons with other membrane proteins. ACS Omega, 2022, 7 (26): 22601-22612.
16. Wang, T.,# Yang, Y.,# He, M., Liu, M., Huang, J.-W., Min, J., Chen, C.-C., Liu, Y.,* Zhang, L.,* Guo, R.-T.* Structural insights into the cyclization of unusual brasilane-type sesquiterpenes. International Journal of Biological Macromolecules, 2022, 209: 1784-1791.
17. Dai, L.,# Zhang, X.,# Hu, Y.,# Shen, J., Zhang, Q., Zhang, L., Min, J., Chen, C.-C., Liu, Y.,* Huang, J.-W.,* Guo, R.-T.* Structural and functional insights into a nonheme iron- and α-ketoglutarate-dependent halogenase that catalyzes chlorination of nucleotide substrates. Applied and Environmental Microbiology, 2022, 88 (9): e0249721.
18. Zeng, W.,# Li, X.,# Yang, Y.,# Min, J.,# Huang, J.-W., Liu, W., Niu, D., Yang, X., Han, X., Zhang, L., Dai, L., Chen, C.-C.,* Guo, R.-T.* Substrate-binding mode of a thermophilic PET hydrolase and engineering the enzyme to enhance the hydrolytic efficacy, ACS Catalysis, 2022, 12(5): 3033-3040.
19. Chen, C.-C.,# Dai, M.,# Zhang, L.,# Zhao, J., Zeng, W., Shi, M., Huang, J.-W., Liu, W., Guo, R.-T.*, Li, A.* Molecular basis for a toluene monooxygenase to govern substrate selectivity, ACS Catalysis, 2022, 12(5): 2831-2839.
20. Mao, Z.,# Xiao, H.,# Shen, P.,# Yang, Y.,# Xue, J., Yang, Y., Shang, Y., Zhang, L., Li, X., Zhang, Y., Du, Y., Chen, C.-C., Guo, R.-T.*, Zhang, Y.* KRAS(G12D) can be targeted by potent inhibitors via formation of salt bridge. Cell Discovery, 2022, 8 (1): 5.
21. Hu, Y.,# Li, H.,# Min, J.,# Yu, Y., Liu, W., Huang, J.-W., Zhang, L., Yang, Y., Dai, L.*, Chen, C.-C.,* Guo, R.-T.* Crystal structure and biochemical analysis of the specialized deoxynivalenol–detoxifying glyoxalase SPG from Gossypium hirsutum. International Journal of Biological Macromolecules, 2022, 200: 388-396.
22. 陈纯琪, 韩旭, 刘卫东, 马立新, 刘珂*，郭瑞庭*. 基于结构改造来源于大阪伊德氏杆菌 201-F6 的PET水解酶，生物工程学报，2021, 37 (9): 3268-3275.
23. Dai, L.,# Chang, Z.,# Yang, J., Liu, W., Yang, Y., Chen, C.-C., Zhang, L., Huang, J.-W.,* Sun, Y.,* Guo, R.-T.* Structural investigation of a thermostable 1,2- β -mannobiose phosphorylase from Thermoanaerobacter sp. X-514. Biochemical and Biophysical Research Communications, 2021, 579: 54-61.
24. Dai, L.,# Qu, Y.,# Hu, Y.,# Min, J., Yu, X., Chen, C.-C., Huang, J.-W.,* Guo, R.-T.* Catalytically inactive lytic polysaccharide monooxygenase PcAA14A enhances the enzyme-mediated hydrolysis of polyethylene terephthalate. International Journal of Biological Macromolecules, 2021, 190: 456-462.
25. Dai, L.,# Qu, Y.,# Huang, J.-W., Hu, Y., Hu, H., Li, S., Chen, C.-C.,* Guo, R.-T.* Enhancing PET hydrolytic enzyme activity by fusion of the cellulose-binding domain of cellobiohydrolase I from Trichoderma reesei. Journal of Biotechnology, 2021, 334: 47-50.
26. Liu, W.,# Ma, C.,# Liu, W.,# Zheng, Y., Chen, C.-C., Liang, A., Luo, X., Li, Z., Ma, W., Song, Y.,* Guo, R.-T.,* Zhang, T.* Functional and structural investigation of a novel β-mannanase BaMan113A from Bacillus sp. N16-5. International Journal of Biological Macromolecules, 2021, 182: 899-909.
27. Chen, C.-C.,# Han, X.,# Li, X.,# Jiang, P., Niu, D., Ma, L., Liu, W., Li, S., Qu, Y., Hu, H., Min, J., Yang, Y., Zhang, L., Zeng, W., Huang, J.-W.,* Dai, L.,* Guo, R.-T.* General features to enhance enzymatic activity of poly (ethylene terephthalate) hydrolysis. Nature Catalysis, 2021, 4(5):425-430. (Highlighted by ChemistryViews, 科技日报, 中国科学报)
28. Chen, C.-C.,# Yu, X.,# Kuo, C.-J.,# Min, J., Chen, S., Ma, L.,* Liu, K.,* Guo, R.-T.* Overview of antiviral drug candidates targeting coronaviral 3C-like main proteases. FEBS Journal, 2021, 228(17): 5089-5121. (Review, https://doi.org/10.1111/febs.15696)
29. Chen, C.-C., Min, J., Zhang, L., Yang, Y., Yu, X.,* Guo, R.-T.* Advanced understanding of the electron transfer pathway of cytochrome P450s. ChemBioChem, 2021, 22(8): 1317-1328. (Review, Cover story)
30. Chen, C.-C.,# Malwal, S. R.,# Han, X.,# Liu, W., Ma, L., Zhai, C., Dai, L., Huang, J.-W., Shillo, A., Desai, J., Ma, X., Zhang, Y., Guo, R.-T.,* Oldfield, E.* Terpene cyclases and prenyltransferases: structures and mechanisms of action. ACS Catalysis, 2021, 11(1): 290-303. (Cover story)
31. Zhang, L.,# Xie, Z.,# Liu, Z.,# Zhou, S., Ma, L., Liu, W., Huang, J.-W., Ko, T.-P., Li, X., Hu, Y., Min, J., Yu, X., Guo, R.-T.,* Chen, C.-C.* Structural insight into the electron transfer pathway of a self-sufficient P450 monooxygenase. Nature Communications, 2020, 11(1): 2676. (Highlighted by C&EN, ChemistryViews, 科技日报, 中国科学报)
32. Chen, C.-C.,* Zhang, L., Yu, X., Ma, L., Ko, T.-P., Guo, R.-T.* Versatile cis-isoprenyl diphosphate synthase superfamily members in catalyzing carbon–carbon bond formation. ACS Catalysis, 2020, 10(8): 4717-4725 (Review, ACS Editors’ Choice, Cover story)
33. Chen, C.-C., Dai, L., Ma, L.,* Guo, R.-T.* Enzymatic degradation of plant biomass and synthetic polymers. Nature Reviews Chemistry, 2020, 4(3): 114-126 (Review, Cover story)
34. Huang, J.-W.,# Niu, D.,# Liu, K.,# Wang, Q., Ma, L., Chen, C.-C., Zhang, L., Liu, W., Zhou, S., Min, J., Wu, S., Yang, Y.,* Guo, R.-T.* Structure basis of non-structural protein pA151R from African Swine Fever Virus. Biochemical and Biophysical Research Communications, 2020, 532(1), 108-113.
35. Liu, Y., Lee, C., Li, F., Trček, J., Bähre, H., Guo, R.-T., Chen, C.-C., Chernobrovkin, A., Zubarev, R., Römling, U.* A Cyclic di-GMP Network Is Present in Gram-Positive Streptococcus and Gram-Negative Proteus Species. ACS Infectious Diseases, 2020, 6(10): 2672-2687.
36. Xiao, X., Elsayed, S. S., Wu, C., van der Heul, H. U., Metsä-Ketelä, M., Du, C., Prota, A. E., Chen, C.-C., Liu, W., Guo, R.-T., Abrahams, J. P., van Wezel, G. P.* Functional and Structural Insights into a Novel Promiscuous Ketoreductase of the Lugdunomycin Biosynthetic Pathway. ACS Chemical Biology, 2020, 15(9): 2529-2538
37. Zhou, S., Ko, T.-P.,* Huang, J.-W., Liu, W., Zheng, Y., Wu, S., Wang, Q., Xie, Z., Liu, Z., Chen, C.-C., * Guo, R.-T.* Structure of a gut microbial Diltiazem-metabolizing enzyme suggests possible substrate binding mode. Biochemical and Biophysical Research Communications, 2020, 527(3): 799-804.
38. Yang, Y., Li, L., Yuan, L., Zhou, X., Duan, J., Xiao, H., Cai, N., Han, S., Ma, X., Liu, W., Chen, C.-C., Wang, L., Li, X., Chen, J., Kang, N., Chen, J., Shen, Z., Malwal, S. R., Liu, W., Shi, Y., Oldfield, E., Guo, R.-T., Zhang, Y. A Structural change in butyrophilin upon phosphoantigen binding underlies phosphoantigen-mediated Vgamma9Vdelta2 T cell activation. Immunity, 2019, 50(4): 1043-1053.
39. Sun, H., Ko, T.-P., Liu, W., Liu, W., Zheng, Y., Chen, C.-C.,* Guo, R.-T.* Structure of an antibiotic-synthesizing UDP-glucuronate 4-epimerase MoeE5 in complex with substrate. Biochemical and Biophysical Research Communications, 2019, 521(1): 31-36.
40. Kuo, C.-J., Gao, J., Huang, J.-W., Ko, T.-P., Zhai, C., Ma, L., Liu, W., Dai, L., Chang, Y.-F., Chen, T.-H., Hu, Y., Yu, X., Guo, R.-T.,* Chen, C.-C.* Functional and structural investigations of fibronectin-binding protein Apa from Mycobacterium tuberculosis. Biochimica et Biophysica Acta. General subjects, 2019, 1863(9): 1351-1359.
41. Tang, X.,# Xue, J.,# Yang, Y., Ko, T.-P., Chen, C.-Y., Dai, L., Guo, R.-T., Zhang, Y.,* Chen C.-C.* Structural insights into the calcium dependence of Stig cyclases. RSC Advances, 2019, 9(23): 13182.
42. Ma, J., Ko, T.-P., Yu, X., Zhang, L., Ma, L., Zhai, C., Guo, R.-T., Liu, W., Li, H., Chen, C.-C.* Structural insights to heterodimeric cis-prenyltransferases through yeast dehydrodolichyl diphosphate synthase subunit Nus1. Biochemical and Biophysical Research Communications, 2019, 515(4): 621-626.
43. Zhang, L., Ko, T.-P., Malwal S. R., Liu W., Zhou S., Oldfield E., Guo R. T.,* and Chen, C.-C.* Complex structures of MoeN5 with substrate analogues suggest sequential catalytic mechanism. Biochemical and Biophysical Research Communications, 2019, 511(4): 800-805.
44. Chang, Z., Ansbacher, T., Zhang, L., Yang, Y., Ko, T.-P., Zhang, G., Liu, W., Huang, J.-W., Dai, L., Guo, R.-T., Major, D. T.,* Chen, C.-C.* Crystal structure of LepI, a multifunctional SAM-dependent enzyme which catalyzes pericyclic reactions in leporin biosynthesis. Organic Biomolecular Chemistry, 2019, 17(8): 2070-2076. (Cover story)
45. Chen, C.-C.,# Hu, X.,# Tang, X.,# Yang, Y.,# Ko, T.-P., Gao, J., Zheng, Y., Huang, J.-W., Yu, Z., Li, L., Han, S., Cai, N., Zhang, Y.,* Liu, W.,* Guo, R.-T.* The Crystal Structure of a Class of Cyclases that Catalyze the Cope Rearrangement. Angewandte Chemie International Edition, 2018, 57(46): 15060-15064. (Cover story)
46. Malwal, S. R., Chen, L., Hicks, H., Qu, F., Liu, W., Shillo, A., Law, W. X., Zhang, J., Chandnani, N., Han, X., Zheng, Y., Chen, C.-C., Guo, R.-T., AbdelKhalek, A., Seleem, M. N., Oldfield, E. Discovery of lipophilic bisphosphonates that target bacterial cell wall and quinone biosynthesis. Journal of Medicinal Chemistry, 2019, 62(5): 2564-2581.
47. Gao, J.,# Ko, T.-P.,# Chen, L.,# Malwal, S. R., Zhang, J., Hu, X., Qu, F., Liu, W., Huang, J.-W., Cheng, Y. S., Chen, C.-C., Yang, Y., Zhang, Y., Oldfield, E., Guo, R.-T.* “Head-to-middle” and “head-to-tail” cis-prenyl transferases: Structure of isosesquilavandulyl diphosphate synthase. Angewandte Chemie International Edition, 2018, 57(3): 683-687. (Cover story)
48. Chen, C.-C.,# Han, X.,# Ko, T.-P., Liu, W.,* Guo, R.-T.* Structural studies reveal the molecular mechanism of PETase. FEBS Journal, 2018, 285(20): 3717-3723.
49. You, S., Chen, C.-C., Tu, T., Wang, X., Ma, R., Cai, H. Y., Guo, R.-T.,* Luo, H. Y.,* Yao, B.*. Insight into the functional roles of Glu175 in the hyperthermostable xylanase XYL10C-ΔN through structural analysis and site-saturation mutagenesis. Biotechnol Biofuels, 2018, 11: 159.
50. Zheng, Y.,# Liu, W.,# Chen C.-C.,# Hu, X., Liu, W., Ko, T.-P., Tang, X., Wei, H., Huang, J.-W., Guo, R.-T.* Crystal Structure of a Mycoestrogen-Detoxifying Lactonase from Rhinocladiella mackenziei: Molecular Insight into ZHD Substrate Selectivity. ACS Catalysis, 2018, 8(5): 4294-4298.
51. Malwal, S. R.,# Gao, J.,# Hu, X.,# Yang, Y.,# Liu, W., Huang J.-W., Ko, T.-P., Li, L., Chen, C.-C., O’Dowd, B., Khade, R. L., Zhang, Y., Zhang, Y., Oldfield, E., Guo, R.-T.* Catalytic role of conserved asparagine, glutamine, serine, and tyrosine residues in isoprenoid biosynthesis enzymes. ACS Catalysis, 2018, 8(5): 4299-4312.
52. Wang, J.,# Chen, C.-C.,# Yang, Y.,# Liu, W., Ko, T.-P., Shang, N., Hu, X., Xie, Y., Huang, J.-W., Zhang, Y.,* Guo, R.-T.* Structural insight into a novel indole prenyltransferase in hapalindole-type alkaloid biosynthesis. Biochemical and Biophysical Research Communications, 2018, 495(2): 1782-1788.
53. Han, X.,# Liu, W.,# Huang, J.-W.,# Ma, J., Zheng, Y., Ko, T.-P., Xu, L., Cheng, Y. S., Chen, C.-C., Guo, R.-T.* Structural insight into catalytic mechanism of PET hydrolase. Nature Communications, 2017, 8(1): 2106.
54. Gao, J.,# Huang, J.-W.,# Li, Q.,# Liu, W., Ko, T.-P., Zheng, Y., Xiao, X., Kuo, C.-J., Chen, C.-C.,* Guo, R.-T.* Characterization and crystal structure of a thermostable glycoside hydrolase family 45 1,4-β-endoglucanase from Thielavia terrestris. Enzyme and Microbial Technology, 2017, 99: 32-37.
55. Hui, R.,# Hu, X.,# Liu, W., Zheng, Y., Chen, Y., Guo, R.-T., Jin, J.,* Chen, C.-C.* Characterization and crystal structure of a novel zearalenone hydrolase from Cladophialophora bantiana. Acta Crystallographica. Section F, Structural Biology Communications, 2017, 73: 515-519.
56. Zhang, L.,# Chen, C.-C.,# Ko, T.-P.,# Huang, J.-W., Zheng, Y., Liu, W., Wang, I., Malwal, S. R., Feng, X., Wang, K., Huang, C.-C., Hsu, S.-T., Wang, A.-H., Oldfield, E.,* and Guo, R.-T.* Moenomycin Biosynthesis: Structure and Mechanism of Action of the Prenyl Transferase MoeN5, Angewandte Chemie International Edition, 2016, 55(15): 4716-4720.
57. Xu, Z.,# Liu, W.,# Chen, C.-C.,# Li Q., Huang, J.-W., Ko, T.-P., Liu, G., Liu, W., Peng, W., Cheng, Y.-S., Chen, Y., Jin, J., Li, H.,* Zheng, Y.,* Guo, R.-T.* Enhanced α‑zearalenol hydrolyzing activity of a mycoestrogen detoxifying lactonase by structure-based engineering. ACS Catalysis, 2016, 6(11): 7657-7663. (Cover story)
58. Liu, M.,# Chen, C.-C.,# Chen, L.,# Xiao, X., Zheng, Y.,Huang, J.-W., Liu, W., Ko, T.-P., Cheng, Y.-S., Feng, X., Oldfield, E.,* Guo, R.-T.,* and Ma, Y.* Structure and function of a “Head-to-Middle” prenyltransferase: pavandulyl piphosphate pynthase. Angewandte Chemie International Edition, 2016, 55(15): 4721-4724. (Cover story)
59. Yan, J.,# Liu, W.,# Li, Y., Lai, H.-L., Zheng, Y., Huang, J.-W., Chen, C.-C., Chen, Y., Jin, J., Li, H.,* Guo, R.-T.* Functional and structural analysis of Pichia pastoris expressed Aspergillus niger 1,4-β-endoglucanase. Biochemical and Biophysical Research Communications, 2016, 475(1): 8-12.
60. Huang, J.-W.,# Liu, W.,# Lai, H.-L., Cheng, Y.-S., Zheng, Y., Li, Q., Sun, H., Kuo, C.-J., Guo, R.-T.,* Chen, C.-C.* Crystal structure and genetic modifications of FI-CMCase from Aspergillus aculeatus F-50. Biochemical and Biophysical Research Communications, 2016, 478(2): 565-572.
61. Yang, Y.,# Ko, T.-P.,# Chen, C.-C.,# Huang, G., Zheng, Y., Liu, W., Wang, I., Ho, M., Hsu, S.-T., O'Dowd, B., Huff, H., Huang, C.-H., Docampo, R., Oldfield, E.,* Guo, R.-T.* Structures of trypanosome vacuolar soluble pyrophosphatases: anti-parasitic drug targets. ACS Chemical Biology, 2016, 11(5): 1362-1371.
62. Zheng, Y.,# Li, Y.,# Liu, W.,# Chen, C.-C., Ko, T.-P., He, M., Xu, Z., Liu, M., Luo, H., Guo, R.-T.,* Yao, B.,* and Ma, Y.* Structural insight into potential cold adaptation mechanism through a psychrophilic glycoside hydrolase family 10 endo-β-1, 4-xylanase. Journal of Structural Biology, 2016, 193(3): 206-211. (Cover story)
63. Liu, G., Li, Q., Shang, N., Huang J. W., Ko, T.-P., Liu, W., Zheng, Y., Han, X., Chen, Y., Chen, C.-C., Jin, J., Guo, R.-T.* Functional and structural analyses of a 1,4- β-endoglucanase from Ganoderma lucidum. Enzyme and Microbial Technology, 2016, 86: 67-74.
64. Hu, Y.,# Liu, W.,# Malwal, S. R., Zheng, Y., Feng, X., Ko, T.-P., Chen, C.-C., Xu, Z., Liu, M., Han, X., Gao, J., Oldfield, E.,* Guo, R.-T.* Structures of iridoid synthase from Cantharanthus roseus with bound NAD(+), NADPH, or NAD(+) /10-oxogeranial: Reaction mechanisms. Angewandte Chemie International Edition, 2015, 54(51): 15478-15482. (Cover story)
65. Haywood, J., Qi, J., Chen, C.-C., Lu, G., Liu, Y., Yan, J., Shi, Y., and Gao, G.-F. Structural basis of collagen recognition by human osteoclast-associated receptor and design of osteoclastogenesis inhibitors. PNAS, 2016, 113(4): 1038-1043.
66. Cheng, Y.-S.,# Chen, C.-C.,# Huang, J.-W., Ko, T.-P., Huang, Z., and Guo, R.-T.* Improving the catalytic performance of a GH11 xylanase by rational protein engineering. Applied Microbiology and Biotechnology, 2015, 99(22): 9503-9510.
67. Zheng, Y.,# Chen, C.-C.,# Ko, T.-P., Xiao, X., Yang, Y., Huang, C.-H., Qian, G., Shao, W.,* and Guo, R.-T.* Crystal structures of S-adenosyl homocysteine hydrolase from the thermophilic bacterium Thermotoga maritima. Journal of Structural Biology, 2015, 190(2): 135-142. (Cover story)
68. Chen, C.-C., Cheng, K.-J., Ko, T.-P., and Guo, R.-T.* Current progresses in phytase research: three-dimensional structure and protein engineering. ChemBioEng Reviews, 2015, 2(2): 76-86. (Review, Cover story)
69. Chen, C.-C., Ko, T.-P., Huang, J.-W., and Guo, R.-T.* Heat- and alkaline-stable xylanases: applications, protein structure and engineering. ChemBioEng Reviews, 2015, 2(2): 95-106. (Review)
70. Chen, Y., Huang, J.-W., Chen, C.-C., Lai, H.-L., Jin, J., Guo, R.-T.* Crystallization and preliminary X-ray diffraction analysis of an endo-1, 4-beta-D-glucanase from Aspergillus aculeatus F-50. Acta Crystallographica. Section F, Structural Biology Communications, 2015, 71: 397-400.
71. Zhu, W., Wang, Y., Li, K., Gao, J., Huang, C.-H., Chen, C.-C., Ko, T.-P., Zhang, Y., Guo, R.-T., Oldfield, E.* Antibacterial drug leads: DNA and enzyme multi-targeting. Journal of Medicinal Chemistry, 2015, 58: 1215-1227.
72. Chen, C.-C.,# Huang, J.-W.,# Zhao, P., Ko, T.-P., Huang, C.-H., Chan, H.-C., Huang, Z., Liu, W., Cheng, Y.-S., Liu, J.-R., and Guo, R.-T.* Structural analyses and yeast production of the β-1,3-1,4-glucanase catalytic module encoded by the licB gene of Clostridium thermocellum. Enzyme and Microbial Technology, 2015, 71: 1-7.
73. Han, X.,# Chen, C.-C.,# Kuo, C.-J., Huang, C.-H., Zheng, Y., Ko, T.-P., Zhu, Z., Feng, X., Wang, K., Oldfield, E., Wang, A.-H., Liang, P.-H., Guo, R.-T.,* and Ma, Y.* Crystal structures of ligand-bound octaprenyl pyrophosphate synthase from Escherichia coli reveal the catalytic and chain-length determining mechanisms. Proteins, 2015, 83(1): 37-45.
74. Cheng, Y.-S.,# Chen, C.-C.,# Huang, C.-H., Ko, T.-P., Luo, W., Huang, J.-W., Liu, J.-R., and Guo, R.-T.* Structural analysis of a glycoside hydrolase family 11 xylanase from Neocallimastix patriciarum: insights into the molecular basis of a thermophilic enzyme. The Journal of Biological Chemistry, 2014, 289(16): 11020-11028.
75. Liu, W.,# Feng, X.,# Zheng, Y.,# Huang, C. H., Nakano, C., Hoshino, T., Bogue, S., Ko, T.-P., Chen, C.-C., Cui, Y., Li, J., Wang, I., Hsu, S. T., Oldfield, E.,* Guo, R.-T.* Structure, function and inhibition of ent-kaurene synthase from Bradyrhizobium japonicum. Scientific Reports, 2014, 4: 6214.
76. Shang, N.,# Li, Q.,# Ko, T.-P.,# Chan, H.-C., Li, J., Zheng, Y., Huang, C.-H., Ren, F., Chen, C.-C., Zhu, Z., Galizzi, M., Li, Z.-H., Rodrigues-Poveda, C. A., Gonzalez-Pacanowska, D., Veiga-Santos, P., de Carvalho, T. M., de Souza, W., Urbina, J. A., Wang, A.-H., Docampo, R., Li, K., Liu, Y.-L., Oldfield, E.,* Guo, R.-T.* Squalene synthase as a target for chagas disease therapeutics. PLoS Pathogens, 2014, 10(5): e1004114.
77. Peng, W.,# Ko, T.-P.,# Yang, Y., Zheng, Y., Chen, C.-C., Zhu, Z., Huang, C.-H., Zeng, Y.-F., Huang, J.-W., Wang, A. H.-J., Liu, J.-R.,* Guo, R.-T.* Crystal structure and substrate-binding mode of the mycoestrogen-detoxifying lactonase ZHD from Clonostachys rosea. RSC Advances, 2014, 4(107): 62321-62325.
78. Huang, C.-H., Zhu, Z., Cheng, Y.-S., Chan, H.-C., Ko, T.-P., Chen, C.-C., Wang, I., Ho, M.-R., Hsu, S. T. -D., Zeng, Y.-F., Huang, Y.-N., Liu, J.-R.,* Guo, R.-T.* Structure and catalytic mechanism of a glycoside hydrolase family-127β-L-Arabinofuranosidase (HypBA1). J Bioprocess Biotech, 2014, 4: 171.
79. Zhang, L.,# Zhao, P.,# Chen, C.-C., Huang, C.-H., Ko, T.-P., Zheng, Y., Guo, R.-T.* Preliminary X-ray diffraction analysis of a thermophilic beta-1,3-1,4-glucanase from Clostridium thermocellum. Acta Crystallographica. Section F, Structural Biology Communications, 2014, 70: 946-948.
80. Xu, J.,# Ren, F.,# Huang, C.-H., Zheng, Y., Zhen, J., Sun, H., Ko, T.-P., He, M., Chen, C.-C., Chan, H.-C., Guo, R.-T.,* Song, H.,* Ma, Y.* Functional and structural studies of pullulanase from Anoxybacillus sp. LM18-11. Proteins, 2014, 82(9): 1685-1693. (Cover story)
81. Wu, T.-H.,# Chen, C.-C.,# Cheng, Y.-S., Ko, T.-P., Lin, C.-Y., Lai, H.-L., Huang, T.-Y., Liu, J.-R., * and Guo, R.-T.* Improving specific activity and thermostability of Escherichia coli phytase by structure-based rational design. Journal of Biotechnology, 2014, 175: 1-6.
82. Lv, P.,# Zhang, L.,# Luo, H., Chen, C.-C., Huang, C.-H., Peng, W., Wang, K., Ko, T.-P., Zheng, Y., Zhang, J., Yao, B.,* Guo, R.-T.* Preliminary X-ray diffraction analysis of thermostable beta-1,4-xylanase from Streptomyces sp. S9. Acta Crystallographica. Section F, Structural Biology Communications, 2014, 70: 105-107.
83. Huang, J.-W.,# Chen, C.-C.,# Huang, C.-H., Huang, T.-Y., Wu, T.-H., Cheng, Y.-S., Ko, T.-P., Lin, C.-Y., Liu, J.-R.,* and Guo, R.-T.,* Improving the specific activity of beta-mannanase from Aspergillus niger BK01 by structure-based rational design. Biochimica et Biophysica Acta, 2014, 1844(3): 663-669. (Cover story)
84. Cheng, Y.-S.,# Huang, C.-H.,# Chen, C.-C., Huang, T.-Y., Ko, T.-P., Huang, J.-W., Wu, T.-H., Liu, J.-R.,* Guo, R.-T.,* Structural and mutagenetic analyses of a 1,3-1,4-beta-glucanase from Paecilomyces thermophila. Biochimica et Biophysica Acta, 2014, 1844(2): 366-373. (Cover story)
85. Chen, C.-C.,# Luo, H.,# Han, X.,# Lv, P., Ko, T.-P., Peng, W., Huang, C.-H., Wang, K., Gao, J., Zheng, Y., Yang, Y., Zhang, J., Yao, B.,* and Guo, R.-T.* Structural perspectives of an engineered beta-1,4-xylanase with enhanced thermostability. Journal of Biotechnology, 2014, 189: 175-182.
86. Ren, F.,# Feng, X.,# Ko, T.-P.,# Huang, C.-H., Hu, Y., Chan, H.-C., Liu, Y.-L., Wang, K., Chen, C.-C., Pang, X., He, M., Li, Y., Oldfield, E.,* Guo, R.-T.* Insights into TIM-barrel prenyl transferase mechanisms: crystal structures of PcrB from Bacillus subtilis and Staphylococcus aureus. ChemBioChem : a European Journal of Chemical Biology, 2013, 14(2): 195-199. (Cover story)
87. Luo, W.,# Huang, J.-W.,# Huang, C.-H., Huang, T.-Y., Chan, H.-C., Liu, J.-R., Guo, R.-T., Chen, C.-C.* Preliminary X-ray diffraction analysis of thermostable beta-1,4-mannanase from Aspergillus niger BK01. Acta Crystallographica. Section F, Structural Biology and Crystallization Communications, 2013, 69: 1100-1102.
88. Li, X.,# Han, X.,# Ko, T.-P., Chen, C.-C., Zhu, Z., Hua, E., Guo, R.-T., Huang, C.-H.* Preliminary X-ray diffraction analysis of octaprenyl pyrophosphate synthase from Escherichia coli. Acta crystallographica. Section F, Structural Biology and Crystallization Communications, 2013, 69: 328-331.
89. Han, X.,# Gao, J.,# Shang, N.,# Huang, C.-H., Ko, T.-P., Chen, C.-C., Chan, H.-C., Cheng, Y.-S., Zhu, Z., Wiegel, J., Luo, W.,*Guo, R.-T.,* Ma, Y.* Structural and functional analyses of catalytic domain of GH10 xylanase from Thermoanaerobacterium saccharolyticum JW/SL-YS485. Proteins, 2013, 81(7): 1256-1265. (Cover story)
90. Lin, C.-W., Chang, Y.-L., Chang, Y.-C., Lin, J.-C., Chen, C.-C., Pan, S.-H., Wu, C.-T., Chen, H.-Y., Yang, S.-C., Hong, T.-M., and Yang, P.-C. MicroRNA-135b promotes lung cancer metastasis by regulating multiple targets in the Hippo pathway and LZTS1, Nature communications, 2013, 4: 1877.
91. Huang, J.-W., Cheng, Y.-S., Ko, T.-P., Lin, C.-Y., Lai, H.-L., Chen, C.-C., Ma, Y., Zheng, Y., Huang, C.-H., Zou, P., Liu, J.-R.,* Guo, R.-T.* Rational design to improve thermostability and specific activity of the truncated Fibrobacter succinogenes 1,3-1,4-beta-D-glucanase. Applied Microbiology and Biotechnology, 2012, 94(1): 111-121.
92. Huang, C.-H.,# Sun, Y.,# Ko, T.-P., Chen, C.-C., Zheng, Y., Chan, H.-C., Pang, X., Wiegel, J.,* Shao, W.,* Guo, R.-T.* The substrate/product-binding modes of a novel GH120 beta-xylosidase (XylC) from Thermoanaerobacterium saccharolyticum JW/SL-YS485. The Biochemical Journal, 2012, 448(3): 401-407.
93. Chan, H.-C.,# Zhu, Y.,# Hu, Y., Ko, T.-P., Huang, C.-H., Ren, F., Chen, C.-C., Ma, Y., Guo, R.-T.,* Sun, Y.* Crystal structures of D-psicose 3-epimerase from Clostridium cellulolyticum H10 and its complex with ketohexose sugars. Protein & Cell, 2012, 3(2): 123-131. (Cover story)
94. Chen, C.-C., Chang, C.-M., Sun, C.-P., Yu, C.-P., Wu, P.-Y., Jeng, K.-S., Hu, C.-P., Chen, P.-J., Wu, J.-C., Shih, C.-H., Gershwin, M. E., and Tao, M.-H. Use of RNA interference to modulate liver adenoma development in a murine model transgenic for hepatitis B virus. Gene therapy, 2012, 19(1): 25-33.
95. Zeng, Y.-F.,# Ko, T.-P.,# Lai, H.-L., Cheng, Y.-S., Wu, T.-H., Ma, Y., Chen, C.-C., Yang, C.-S., Cheng, K.-J., Huang, C.-H., Guo, R.-T.,* Liu, J.-R.* Crystal structures of Bacillus alkaline phytase in complex with divalent metal ions and inositol hexasulfate. Journal of Molecular Biology, 2011, 409(2): 214-224. (Cover story)
96. Wu, T.-H.,# Huang, C.-H.,# Ko, T.-P., Lai, H.-L., Ma, Y., Chen, C.-C., Cheng, Y.-S., Liu, J.-R.,* Guo, R.-T.* Diverse substrate recognition mechanism revealed by Thermotoga maritima Cel5A structures in complex with cellotetraose, cellobiose and mannotriose. Biochimica et Biophysica Acta, 2011, 1814(12): 1832-1840. (Cover story)
97. Chen, C.-C., Sun, C.-P., Ma, H.-I., Fang, C.-C., Wu, P.-Y., Xiao, X., and Tao, M.-H. Comparative study of anti-hepatitis B virus RNA interference by double-stranded adeno-associated virus serotypes 7, 8, and 9. Molecular therapy : the journal of the American Society of Gene Therapy, 2009, 17(2): 352-359.
98. Chen, C.-C., Ko, T.-M., Ma, H.-I., Wu, H.-L., Xiao, X., Li, J., Chang, C.-M., Wu, P.-Y., Chen, C.-H., Han, J.-M., Yu, C.-P., Jeng, K.-S., Hu, C.-P., and Tao, M.-H. Long-term inhibition of hepatitis B virus in transgenic mice by double-stranded adeno-associated virus 8-delivered short hairpin RNA, Gene therapy, 2007, 14(1): 11-19.
六、已授权专利
1. PET hydrolase having improved thermal stability，美国，US11680252，1/10
2. 具有提升的热稳定性的PET降解酶，中国，CN115125225B，1/10
3. 具提升活性的PET水解酶，中国，CN114480343B，1/11
4. 具提升酶活性的纤维素酶，美国，US10479985B1，1/12
5. 酶活提升的纤维素酶，中国，CN111826367B，1/10
6. 具有提升的酶活性的植酸酶，中国，CN104342418B，2/8
7. 具有提升的酶活性的植酸酶，中国，CN106399273B，2/8
8. Cellulase having improved enzymatic activity，美国，US9809807B1，2/8
9. Phytase having improved thermostability，美国，US10011822B2，7/8
10. Glucose oxidase having improved thermostability，美国，US10011822B2，9/10
11. Lactone hydrolase and method of degrading alpha-zearalenol using the same，美国，US10316303B2，4/8
12. Lysozyme having improved enzymatic activity，美国，US10323237B2，1/4
13. Xylosidase having improved enzymatic activity，美国，US20190119687A1，9/10
14. Cellulase having improved enzymatic activity，美国，US16406255，1/11
七、获奖与荣誉称号
1. 2020年，湖北省创新群体
2. 2020年，湖北省省级人才入选者
3. 2020年，天津市自然科学二等奖（第二完成人）
获选的其中16篇封面文章
