一、基本信息

王飞，博士，副教授

电子邮件：wangfei(AT)hubu.edu.cn

研究方向：合成生物学，绿色生物制造，基因工程，新型可编程核酸酶的挖掘与应用研究

社会兼职：湖北省生物工程学会会员

二、教育经历

2011.09–2016.06  湖北大学，生化与分子生物学，博士

2008.09–2011.06  湖北大学，理学硕士学位

2004.09–2008.06  武汉工程大学，工学学士学位

三、工作经历

2021.7至今      九游会老哥俱乐部   讲师、副教授

2017.06–2021.06  九游会老哥俱乐部，博士后

2016.06–2017.05  武汉中拓康明生物科技有限公司重组疫苗&核酸疫苗研发主管

四、发表论文（#并列一作，*通讯作者）：

1.        Liu Y, Li, W, Jiang X, Wang Y, Zhang Z, Liu Q, He R, Chen Q, Yang J, Wang L, Wang F^*, Ma L^*. (2021) A programmable omnipotent Argonaute nuclease from mesophilic bacteria Kurthia massiliensis. Nucleic Acids Research 49, 1597–1608.

2.        Wang L, He R, Lv B, Yu X, Liu Y, Yang J, Li W, Wang Y, Zhang H, Yan G, Mao W, Liu L^*, Wang F^*, L Ma L^*. (2021) Pyrococcus furiosus Argonaute coupled with modified ligase chain reaction for detection of SARS-CoV-2 and HPV. Talanta 227, 122154.

3.        Wang F#, Yang J#, He R#, Yu X#, Chen S, Liu Y, Wang L, Li A, Liu L, Zhai C, Ma L. (2020) PfAgo-based detection of SARS-CoV-2. Biosensors and Bioelectronics 177, 112932.

4.        He R, Wang L, Li J, Ma L^*, Wang F^*, Wang Y^*. (2020) Integrated analysis of a competing endogenous RNA network reveals a prognostic signature in kidney renal papillary cell carcinoma. Frontiers in Cell and Developmental Biology 8, 612924.

5.        Liu M#, Wang Bin#, Wang F#, Yang Z, Gao D, Zhang C, Ma L*, Yu X*. (2019) Soluble expression of single-chain variable fragment (scFv) in Escherichia coli using superfolder green fluorescent protein as fusion partner, Applied Microbiology and Biotechnology 103, 6071-6079.

6.        Wang F# , Chen Q#, Li S, Zhang C, Li S, Liu M, Mei K, Li C, Ma L*, Yu X. Linear DNA vaccine prepared by large-scale PCR provides protective immunity against H1N1 influenza virus infection in mice, Veterinary Microbiology, 2017, 205, 124-130.

7.        Wang F, Wang X, Yu X, Fu L, Liu Y, Ma L*, Zhai C*. (20) High-level expression of endo-β-N-acetylglucosaminidase H from Streptomyces plicatus in Pichia pastoris and its application for the deglycosylation of glycoproteins, Plos One, 2015, 10: e0120458.

8.        Wang F, Li S, Zhao H, Bian L, Chen L, Zhang Z, Zhong X, Ma L*, Yu X*. (2015) Expression and characterization of the RKOD DNA polymerase in Pichia pastoris, PLoS One 10, e0131757.

9.        Wang L, Qin W, Xu W, Huang F, Xie X, Wang F, Ma L, Zhang C. (2021) Bacteria-mediated tumor therapy via photothermally-programmed cytolysin A expression. Small 17, e2102932.

10.    Hu YH, Wang Y, Wang F, Dong YM, Jiang WL, Wang YP, Zhong X, Ma LX. (2021) SPOP negatively regulates Toll-like receptor-induced inflammation by disrupting MyD88 self-association. Cellular & Molecular Immunology 18, 1708–1717.

11.    He R, Wang L, Wang F, Li W, Liu Y, Li A, Wang Y, Mao W, Zhai C*, Ma L*, (2019) Pyrococcus furiosus Argonaute-mediated nucleic acid detection. Chemical Communications (Camb) 55, 13219-13222.

12.    She W, Ni J, Shui K, Wang F, He R, Xue J, Reetz MT, Li A, Ma L. (2018) Rapid and error-free site-directed mutagenesis by a PCR-free in vitro CRISPR/Cas9-mediated mutagenic system. ACS Synthetic Biology 7, 2236-2244.

13.    Sun K, Li L, Yu X Li L, Meng Q, Wang F, et al. (2017) Functionalization of mixed ligand metal-organic frameworks as the transport vehicles for drugs. Journal of Colloid & Interface Science 486, 128-135.

14.    Tang W, Li Z, Li C, Yu X, Wang F, Wan X, Wang Y, Ma L. (2016) High-level expression and characterization of the Bacillus subtilis subsp. subtilis str. BSP1 YwaD aminopeptidase in Pichia pastoris. Protein Expression and Purification 122, 23-30.

15.    Li M, Yu X, Wang F, Zhai C, Shen W, Yu X, et al. (2015). Secretory expression of organophosphorus hydrolase ophc2 in yarrowia lipolytica polg. Journal of Environmental Science & Health Part B 50, 691-697.

16.    Zhang, X., Liu, J., Yu, X., Wang, F., Li, Y., & Li, Z., et al. (2015). High-level expression of human arginase i in pichia pastoris and its immobilization on chitosan to produce l-ornithine. BMC Biotechnology 15, 1-10.

17.    Shu M, Shen W, Wang X, Wang F, Ma L, Zhai C. (2015) Expression, activation and characterization of porcine trypsin in Pichia pastoris GS115. Protein Expression and Purification 114, 149-155.

18.    Zhong X, Zhai C, Yang D, Jiang S, Li Z, Yu X, et al. Zhong X, Zhai C, Yang D, Jiang S, Li Z, Yu X, Chen L, Zhang Z, Wang F, Wang Y, Chen W, Ma L. (2013) A single-step mixing cloning method for assembly of lentiviral short hairpin RNA expression vectors for gene silencing. Analytical Biochemistry 438, 39-41.

19.    余晓岚，李玉，王飞，钟星，陈亮，马立新*. Preparation of Monoclonal Antibodies Against KOD DNA Polymerase.化学与生物工程，2010，3：63-65.

20.    梅堃，王飞，张晨瑶，左江，余晓岚*.SIRT1的研究进展.现代生物医学进展，2015， 15（14）：2383-27897.

五、授权专利

1.        专利名称：重组CL7-CVN蛋白及其制备方法与应用；发明人：余晓岚，王斌，王飞，马立新，杨智，刘敏，高丹，陈冠军；授权日期：2021.05.04

2.        专利名称：一种SARS-CoV-2病毒核酸检测的引物组、试剂盒及其应用；发明人：马立新，王飞，何如怡，翟超，杨军，刘洋，余晓，刘琳琳；授权日期：2021.08.23